'Catalase Lab Report\r'

' investigate the effect of different environmental factors on the reply of colored and hydrogen peroxide Table of limit 1 Design3 1. 1 Variables3 1. 2 Safety and Environment3 2 Data Collection and Analysis3 2. 1 Collected in the buff Data3 2. 2 Qualitative data5 2. 3 Processed Data5 2. 4 Graph on screen supply 25 2. 5 Graph on hear thermionic tube 36 2. 6 Errors6 3. Conclusion and Evaluation6 3. 1. Conclusion6 3. 2. Evaluation7 3. 2. 1. random Errors7 3. 2. 2. authoritative Errors7 3. 3. Improvements7 Design Look to sheet titled: ‘investigation the action of the enzyme catalase 1 1. 1 Variables The independent variables atomic number 18 the acids used, the dependant variables the height of the bubbles formed and the control variables the test tubes used. 2 1. 2 Safety and Environment The refuge and environmental precautions for this lab are quite strict. raid safety goggles and a lab surface to avoid getting any acid on your person, and dispose of the acids in a sink with galvanic pile of water.\r\nCollect the used liver samples and dispose of accordingly. 2 Data Collection and Analysis 1 2. 1 Collected Raw Data |Test tube |Height of bubbles (cm) | | | | |pH | | |30s | 30s |60s |90s |120s |150s |180s |210s |240s |270s |300s | |1 |0 |0 |0 |0 |0 |0 |0 |0 |0 |0 | |2 |12,9 |13 |14,1 |13,3 |11,7 |10,4 |9,3 |6,7 |5,5 |5 | |3 |14,2 |10,8 |8,4 |6,9 |5,5 |7,5 |3,3 |3 |2,8 |2,7 | |4 |1,9 |1,9 |1,8 |1,4 |1,3 |1,3 |0,4 |0,2 |0,1 |0,1 | |5 |0,2 |0,2 |0,2 |0,1 |0,1 |0,1 |0,1 |0,1 |0,1 |0,1 | |6 |0,5 |0,6 |0,9 |1 |1,4 |1,6 |1,8 |2 |1,3 |1,4 | | 2 2. 4 Graph on test tube 2 3 4 2. 5 Graph on test tube 3\r\nAs mickle be seen from the two above graphs, test tube 3, which had the exact same conditions as test tueb 2 except for the surface heavens of the liver, had a practically more vigourous reaction, due(p) to the increased surface area of the crushed liver. 2. 6 Errors The only piece of equipment that is to be considered in error calculations is the ruler used which had an incredulity of approx. ±0,2 cm. 3. Conclusion and Evaluation 3. 1. Conclusion The experiment was meant to grow an artificial catalase reaction between the enzymes in the liver sample and hydrogen peroxide.\r\nThe sample in test tube 2 reacted with the hydrogen peroxide and produced type O bubbles at a somewhatwhat steady pace, plot of land the sample in test tube 3 reacted vigorously at first and then slowed pass as most of the enzyme had been used up. 3. 2. Evaluation 3. 2. 1. Random Errors The liver was quite hard to get into utter(a) ? cm3 cubes, as it tended to get squished when the knife was pressed down, qualification the sample larger lengthwise, but smaller height-wise.\r\n as well as when the reaction was very rigorous, the oxygen bubbles sometimes elevate the liver out of the hydrogen peroxide, causing it to cede reacting with the hydrogen peroxide. While not a major issue, sometimes the time at which the results were canvass were not exactly at the designated 30 second intervals, due to many things going on at once. 3. 2. 2. opinionated Errors The ruler we were using was quite old and dirty, with some of the finer millimeter markings rubbed off or obscured, hint to readings that were not as accurate as they could apply been.\r\nThe yard of the chemicals used can also be put into question. 3. 3. Improvements The liver could be frozen or in some opposite way petrified to confine the cutting easier and more precise. The liver should be checked constantly and adjusted back down with a glass rod if necessary. Enough time should be allocated to ensure that the experiment can be do in a calm and orderly expressive style to avoid any oversights in the time taking. just and clear rulers should be used to measure the bubbles. The molarity of the chemicals should be checked with titration or some other form of double checking the molarity.\r\n'